MY MISSION: TO PROVIDE AFFORDABLE AND EFFECTIVE COACHING AND TREATMENTS FOR THE AVERAGE AMERICAN THAT ACTUALLY MAKES YOU HEALTHIER AND PROSPEROUS

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If you have ever contemplated getting regenerative stem cell and/or Exosome therapy but felt like you couldn’t afford it… I have the solution!

First let me state that YES you can get regenerative therapy by an FDA IND approved clinic in the United States. You DO NOT need to travel to Panama or Mexico for therapy that is ridiculously expensive with many add-on procedures that cost you time and money and have very little, if any, benefits.

Their Treatment Protocol: 120 million expanded allogenic (Expanded Allogenic Stem Cells are illegal in the USA because cells are being cloned and may cause adverse effects) mesenchymal stem cells (Intravenous administration). Treatment length three days Included in the package: Complimentary VIP airport arrival service for two adults…Complimentary Airport transportation service for two adults …Complimentary 4-night stay at Hilton Panama with breakfast and Wi-Fi (for two adults)…Treatment-related, laboratory services, Treatment-related medical services and facilities. COST: 26,900.00 US Dollars!!! (DOES NOT INCLUDE YOUR AIRFARE AND TRAVEL EXPENSES).

Our Treatment Protocol: 100 million allogenic mesenchymal stem cells + 3 trillion exosomes (Intravenous and local injections) … No travel necessary in the USA. All treatment can be done via telemedicine and with an M.D. or Nurse in your area that we will assign through our network…COST: $3900.00-$7000.00 and We provide an installment plan for those that cannot afford the full amount up front.

FREQUENTLY ASKED QUESTIONS

Will cord blood stem cell therapy help my knee, hip, foot & shoulder pain?

With a third of the US suffering from chronic pain, and over a million each year undergoing joint

replacements in the US, stem cell therapy may eliminate your pain and increase your mobility.

Some patients experience a huge reduction in pain within weeks of one injection. Previous

treatments have included steroid injections, opioid (narcotic) pain / non-steroidal anti -

inflammatory medications and even joint replacement. Stem cell therapy can repair fibrous

connective tissue and stimulate the formation of cartilage, tendon, ligaments & bone. Cord blood

stem cells not only promotes long term pain relief but a significant restoration of normal functions.

Northeast Spine & Wellness Center now offers a cutting - edge regenerative medicine / Stem Cell

therapy treatment option.

How do cord blood stem cell injections work?

Cord blood cell injections offer the ability to heal damaged tissue naturally. Stem cells may repair

the body by dividing to replenish cells that are damaged by disease, injury, or normal wear. The

array of benefits related to cord blood is supported through the successful transplantation and

coordination of multiple components. Umbilical cord blood contains a beneficial blend of

cytokines, growth factors and stem cells that ensure a rich microenvironment to foster tissue

regeneration and cellular growth. Your body has the natural ability to heal itself with stem cells

and other derivatives. As you get older, the amount of stem cells you have in your body are greatly

reduced and are not as efficient. Stem cells have emerged as a key element of regenerative

medicine therapies. They also have immune - modulation properties which are very helpful in

controlling pain & inflammation in the body. Regenerative medicine therapies include natural

healing properties - reduces pain & inflammation - increase blood flow - minimally invasive -

outpatient treatment - no down time - non-steroidal - safe and effective - promotes soft tissue

growth - no adverse reactions.

What benefits does cord blood stem cell therapy offer?

Stem Cell therapy is an option for those looking for an alternative to surgery or potentially total

joint replacement. Regenerative medicine and stem cell therapy allows a human body to naturally

heal. It stimulates healthy cell growth and regenerates healthy tissue. Stem cell therapies have a

multitude of growth factors producing cells. Growth factors are proteins naturally found in the

body that promote the growth of cells and tissues. Stem cell therapies also use interleukins anti -

inflammatory that reduce swelling and inflammation. Regenerative medicine therapy can stimulate

your body’s own stem cells to proliferate and target damaged cells in your body and repair or

replace them. Want to avoid surgery and down time? Do you suffer from back pain - knee injuries

- knee pain - osteoarthritis - tendon / ligament / cartilage tears & injuries - muscle tears - arthritis

- back pain - golfer's elbow - tennis elbow - joint injuries - hip pain - shoulder pain - rotator cuff

injury - carpal tunnel syndrome - plantar fasciitis ...? Then regenerative medicine therapies are for

you!

Are cord blood stem cell injections safe?

Cord blood stem cell therapy is extremely safe and effective. The injections / therapies have been

used more than 60,000 times in the United States with no reported adverse side effects and with

100% safety record in Europe with 100,000 patients treated. Every donor is tested under infectious

disease panels that surpass governmental standards. All cord blood stem cell donors go through a

rigorous screening process, as determined by the Food and Drug Administration (FDA) and

American Association of Tissue Banks (AATB). Furthermore, cord blood stem cells are fully

documented, and screen tested thoroughly according to FDA's registry requirements. This ensures

the optimum quality and potent stem cells for a premium application. Regenerative medicine

therapies are non - surgical procedures that take a minimal amount of time, faster recoveries,

completely natural and no need to take pain medications. Regenerative medicine therapies use

stem cells and their derivatives to help heal, regenerate tissue and decrease inflammation to

improve your quality of life.

Am I a candidate for stem cell therapy?

You may be a good candidate for stem cell therapy if you have been suffering from joint pain and

want to improve your quality of life while avoiding complications related to invasive surgical

procedures. Regenerative medicine is now the easiest noninvasive method to improve your quality

of life. Patients suffering with knee, hip, shoulder, joint pain, etc. may be considered as candidates

for cord blood stem cell therapy. If you have already tried conventional treatments, including

steroids, narcotic pain & anti - inflammatory medications, etc.., cutting - edge regenerative

medicine / Stem Cell therapy is a safe and effective option. Call to schedule a consultation with

one of our doctors to determine if cord blood stem cell therapy is right for you.

AlocyteTM _ UCB Derived

Allogenic Umbilical Cord Blood Plasma combined with Mononuclear Cells (UCB-PM)

• Investigational New Drug (ClinicalTrials.gov ID NCT05909709)

• Authorized by FDA on April 29th, 2022 verifies that Alocyte contains minimum of 100 million stem cells and 3 trillion exosomes.

*Investigational New Drug authorization by FDA is done only after intense scrutiny and testing to prove Safety and efectiveness.

Description:

• UCB-PM is an allogeneic, minimally manipulated product derived from allogenic umbilical cord blood collected from normal healthy planned cesarean section donors. We have completed extensive characterization of the final UBP-PM product to elucidate the regenerative compartments mediating our observed therapeutic effect.

• Alocyte’s manufacturing methodology is designed to preserve the naturally-occurring soluble proteins and nanoparticles including exosomes present in full-term cord blood UCB-PM. Therefore, we have characterized these two distinctly and therapeutically important components.

• There is no addition or combination of any other substance of diluent to the Alocyte product during production besides the cryopreservation solution dimethyl sulfoxide (DMSO)

• Each 1.0 ml Vial of Alocyte is guaranteed to have > 100 million Stem Cells and > 3 Trillion Exosomes

QCM Biologics products are distributed for analytical testing and/or research use purposes and/or use with an FDA approved Investigational New Drug (IND) application, or other Institutional Review Board (IRB) approved protocols or similar. The use with patients should include full disclosure and a signed consent form.

Alocyte is derived from Umbilical Cord Blood includes mononuclear stem cells and a large volume of exosomes.

Alocyte contains proteins, lipids, carbohydrates, and genetic material including exosomes and Stem Cells.

It Includes miRNA and mRNA, which can be used to regulate gene expression in cells.

In 3rd Party testing, Alocyte shows several trillion exosomes per ml with an optimal balance of surface proteins.

Not all extracellular vesicles "EV” are true exosomes. They are between 30-150 nm and require a specific profile. Larger EVs are non-regenerative microvesicles and “apoptotic bodies” produced by dying cells.

We use a proprietary method for filtering micronized material. Our retained EV's have the proper balanced profile of surface proteins CD9, CD63, and CD81 verifying these are true exosomes.

IMPORTANT NOTICE: QCM Biologics products are distributed for analytical testing and/or research use purposes and/or use with an FDA approved Investigational New Drug (IND) application, or other Institutional Review Board (IRB) approved protocols or similar. The use with patients should include full disclosure and a signed consent form.

WHITE PAPER

JWLudlow Consulting, LLC Mobile: 919-623-4221

EIN: 46-0753696 Email: ludlow.johnw@gmail.com

White Paper (Extract)

Concentration, size, and marker protein expression of exosomes isolated from amniotic fluid and umbilical cord blood

John W. Ludlow, Ph.D.

Executive Summary:

A single lot of exosomes isolated from amniotic fluid and umbilical cord blood, provided by the Center for Regenerative Medicine Laboratories, located at 1001 North east 125th street, Miami, FL. 33161, was analyzed in triplicate. The mean and mode sizes of the particles showed consistency within each the lot for the triplicate samples. Particle size for all samples is the size expected for exosomes. Similarly, particle concentration within each lot is consistent. To confirm that these particles are indeed exosomes,

surface protein analysis for CD9, CD63, and CD81 was performed. The relative amounts of CD9, CD63, and CD81 showed consistency within each lot for the triplicate samples. The conclusion here is that the manufacturing process is robust and large quantities (trillions) of exosome particles are obtained per dosage unit of 1mL.

Introduction:

The term exosome is generally understood to reference a specific class of lipid-membrane bound extracellular vesicle (EV) characterized by a diameter of 40–150 nm and a density of 1.09–1.18 g/ml. Exosomes participate in a variety of cellular activities and have been shown to be isolatable from multiple body fluids including saliva, urine, plasma, serum, breast milk and amniotic fluid, as well as from the conditioned media of cultured cells (1, 2). In fact, exosomes can be isolated from any cell type which can be cultured. Purified exosomes have been demonstrated to have clinically relevant therapeutic bioactivity across multiple in vitro and in vivo models (3). In this white paper, a single lot of exosomes isolated from amniotic fluid and a single lot isolated from

umbilical cord blood, provided by the Center for Regenerative Medicine Laboratories, located at 1001 North east 125th street, Miami, FL. 33161, was each analyzed in triplicate. The purpose for this analysis was to determine the quantity of particles which may be isolated from a given dosage sample when processed by standard methods previously shown to isolate exosomes. Analysis of particle size and surface protein expression of these isolated particles was also undertaken to confirm that the particles were indeed exosomes.

Methods:

A 1mL sample of frozen amniotic fluid or umbilical cord blood was thawed and diluted to 12mL with sterile Dulbecco’s phosphate buffered saline (DPBS) prior to centrifugation at 3000xg for 20min to pellet large microsomes and cellular debris. This clarified supernate was then centrifuged at 100,000xg for 2hr to

pellet the exosome particles. The supernate was aspirated and discarded, and the resulting pellet resuspended in 1mL of sterile DPBS.

Particle quality was assessed using a Thermo NanoDrop spectrophotometer for protein determination and approximate RNA concentration by direct absorbance; exosome particles were not lysed, stained. Particle diameter and concentration was measured by Nanoparticle Tracking Analysis (NTA) using a Particle Metrix ZetaView®.

Particle surface protein analysis was carried out using Milteny’s MACSPlex Exosome Kit, human (Cat# 130-108-813).

Conclusion:

The manufacturing process followed by the Center for Regenerative Medicine Laboratories is robust, resulting in large quantities (trillions) of exosome particles contained in a 1mL dose.

References:

1. Thery C, Clayton A, Amigorena S, et al. Isolation and characterization of exosomes from cell culture

supernatants and biological fluids. Curr Prot Cell Biol. 2006;3.22.1– 3.22.29.

DOI:10.1002/0471143030.cb0322s30.

2. Whitford W, Ludlow JW, Cadwell JJS. Continuous production of exosomes. Gen Engin & Biotechnol

New

3. Basu J, Ludlow JW. Exosomes for repair, regeneration and rejuvenation. Expert Opin Biol Ther.

2016;16(4):489-506. doi: 10.1517/14712598.2016.1131976. Review. PMID: 26817494.s 35 (16): 34,

2015.

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AlocyteTM _ UCB Derived